Efficient production of polymer-grade L-lactic acid from corn stover hydrolyzate by thermophilic Bacillus sp. strain XZL4
نویسندگان
چکیده
Lactic acid has been identified as one of the top 30 potential building-block chemicals from biomass. Therefore, the search for cheap raw materials is an objective to reduce the production costs. Efficient polymer-grade L-lactic acid production was achieved in this report by a thermophilic strain Bacillus sp. XZL4 using corn stover hydrolyzate as sole carbon source. High L-lactic acid concentration (81.0 g L(-1)) was obtained from 162.5 g L(-1) concentrated corn stover hydrolyzate (total reducing sugar of 83.0 g L(-1)) with a volumetric productivity of 1.86 g L(-1) h(-1) (0-36 h) and a product yield of 0.98 g g(-1) total reducing sugars. This is the highest L-lactic acid concentration and yield reported from corn stover hydrolyzate. And the high optical purity of L-lactic acid obtained in this study also indicated that Bacillus sp. XZL4 is a promising polymer-grade L-lactic-acid producer from cellulosic biomass.
منابع مشابه
Jerusalem artichoke powder: a useful material in producing high-optical-purity l-lactate using an efficient sugar-utilizing thermophilic Bacillus coagulans strain.
Jerusalem artichoke is a low-requirement crop, which does not interfere with food chain, and is a promising carbon source for industrial fermentation. Microbial conversion of such a renewable raw material to useful products, such as lactic acid, is an important objective in industrial biotechnology. In this study, high-optical-purity l-lactate was efficiently produced from the hydrolysates of J...
متن کاملNon-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6
BACKGROUND The demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (PLA), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. Optically pure L-lactic acid is essential for polymerization of PLA. The high fermentation cost of L-lactic acid is another limitation for PLA polymers...
متن کاملEfficient Open Fermentative Production of Polymer-Grade L-Lactate from Sugarcane Bagasse Hydrolysate by Thermotolerant Bacillus sp. Strain P38
Lactic acid is one of the top 30 potential building-block chemicals from biomass, of which the most extensive use is in the polymerization of lactic acid to poly-lactic-acid (PLA). To reduce the cost of PLA, the search for cheap raw materials and low-cost process for lactic acid production is highly desired. In this study, the final titer of produced L-lactic acid reached a concentration of 185...
متن کاملLactic Acid Production from Pretreated Hydrolysates of Corn Stover by a Newly Developed Bacillus coagulans Strain
An inhibitor-tolerance strain, Bacillus coagulans GKN316, was developed through atmospheric and room temperature plasma (ARTP) mutation and evolution experiment in condensed dilute-acid hydrolysate (CDH) of corn stover. The fermentabilities of other hydrolysates with B. coagulans GKN316 and the parental strain B. coagulans NL01 were assessed. When using condensed acid-catalyzed steam-exploded h...
متن کاملRepeated open fermentative production of optically pure L-lactic acid using a thermophilic Bacillus sp. strain.
A thermophilic Bacillus sp. strain 2-6 was used in completely open repeated batch fermentation for producing optically pure L-lactic acid. Up to 107 g l(-1) L-lactic acid of optical purity 99.8% was obtained with NaOH as pH regulator. Unexpectedly, accumulated cells did not necessarily lead to increased L-lactic acid production. Kinetic and viable cells analyses revealed that L-lactic acid prod...
متن کامل